Composite

Part:BBa_K539655:Design

Designed by: Shu-Han Chang   Group: iGEM11_NCTU_Formosa   (2011-09-27)

a RBS (B0034)+ilvD with RNA thermometer+tetR+terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1739
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2628
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1525
    Illegal BsaI site found at 1782


Design Notes

Built in strong expressing RBS, ilvD (Part:BBa_K539626), 37’celcius regulator RBS, tetR and terminator (Part:BBa_K332033).The sequence of ilvD had been mutated to delet the function of RE site at the origin so that we can submit this part to the iGEM.


Source

The sequence of ilvD comes from Escherichia coli str. K-12 substr. MG1655 .And the part which repress Ptet when the temperature up to 37'Celius is designed by 2010 iGEM NCTU_Formosa.

References